杂色云芝预处理杨木刨花制备纤维板的初步研究

 2023-08-01 06:08

论文总字数:18825字

摘 要

醛基胶黏剂的使用导致传统纤维板在制备及使用过程中产生甲醛等有毒气体释,严重危害人体健康。故此,制备绿色无害的纤维板就成为了目前的热点。本论文以杨木刨花为原料,利用杂色云芝预处理,获得不额外添加化学胶黏剂的纤维板,并初步探讨生物预处理实现纤维板无胶粘黏的作用机制。研究发现,杨木刨花在35d的预处理时间内,纤维板强度总趋势为递增,在第35d达到最高值25.79±1.72MPa;而多糖含量持续降低,35d时达到最低值0.21mg/g;还原糖含量则是先升高再降低并趋于稳定,14d时达到最高值2.12mg/g;通过对木质素降解酶活性的测定,漆酶及木质素过氧化物酶活性暂时未能测出,锰过氧化物酶活性的总趋势是增加,21d略有降低,在第28d达到最高值944.91U/g.min并开始降低;通过对三种木质纤维组分含量的测定,木质素含量的变化趋势与弯曲强度的变化趋势相吻合,与纤维板的强度呈正相关;纤维素含量基本不变;半纤维素则是先快速降低,在第21d开始缓慢降低,与纤维板的强度呈负相关。本论文的研究,将为各类木质纤维原料(尤其是杨木刨花)的纤维板研制提供理论和技术支持。

关 键 词:杂色云芝,生物预处理,杨木刨花,纤维板,弯曲强度

Abstract: The use of aldehyde based adhesives leads to the release of toxic gases such as formaldehyde in the process of preparation and use of traditional fiberboards, which seriously endanger human health. Therefore, the preparation of green and harmless fiberboard has become the current hot spot. In this paper, poplar wood shaving is used as the raw material and pretreated with Coriolus versicolor, the fiberboard is obtained without additional chemical adhesive, and the mechanism of the biological pretreatment to realize the non adhesive viscosity of the fiberboard is preliminarily discussed. The study found that the general trend of fiber plate strength increased gradually in the preprocessing time of 35d, and reached the highest value of 25.79±1.72MPa in 35d, while the content of polysaccharide continued to decrease and 35d reached the minimum value 0.21mg/g; reducing sugar content was first increased and then decreased and stabilized, and 14d reached the highest value 2.12mg/g; through to wood. It was found that the activity of laccase and lignin peroxidase could not be measured, and the total trend of the activity of Mn peroxidase was increasing, reaching the highest value of 944.91U/g.min and decreasing from the 28d; the change trend of lignin content and the change of flexural strength by the determination of the content of three kinds of wood fiber components. The trend is consistent with the strength of the fiberboard, and the cellulose content is basically unchanged, and the hemicellulose is rapidly reduced first and slowly decreases at the beginning of 21d, and has a negative correlation with the strength of the fiberboard. The research in this paper will provide theories and technical support for the development of Fiberboard Materials for all kinds of lignocellulosic materials, especially poplar wood shavings.

Keywords: Coriolus versicolor,biological pretreatment, poplar wood shavings, fiberboard, bending strength

目录

1. 前言 6

1.1纤维板的发展状况 6

1.1.1我国木材资源现状以及合理利用原料 6

1.1.2纤维板中的甲醛污染 6

1.2生物预处理方法制造纤维板技术 7

1.2.1无胶纤维板的研究现状 7

1.2.2 利用生物酶生产无胶纤维板的研究 7

1.2.2利用白腐菌生产无胶纤维板的研究 8

1.3杨木刨花 8

1.4杂色云芝 8

1.5研究思路 8

2.材料与方法 10

2.1材料 10

2.1.1菌种 10

2.1.2杨木刨花 10

2.1.3试剂 10

2.1.4培养基 11

2.1.5溶液 11

2.1.6仪器设备 11

2.2方法 12

2.2.1固体发酵方法 12

2.2.2纤维板压制方法 12

2.2.3纤维板强度测定方法 12

2.2.4漆酶酶活测定方法 12

2.2.5木质素过氧化物酶活测定方法 13

2.2.6锰过氧化物酶活测定方法 13

2.2.7多糖含量测定方法 13

2.2.8还原糖含量测定方法 14

2.2.9木质素含量测定方法 15

2.2.10半纤维素含量测定方法 16

2.2.11纤维素含量测定方法 17

3. 结果与分析 18

3.1预处理不同时间对纤维板强度的影响 18

3.2杂色云芝代谢产物分析及其与纤维板强度的关联度比较 18

3.2.1多糖含量分析及其与纤维板强度的关联度比较 18

3.2.2还原糖含量分析及其与纤维板强度的关联度比较 19

3.2.3漆酶活性分析及其与纤维板强度的关联度比较 20

3.2.4木质素过氧化物酶活性分析及其与纤维板强度的关联度比较 20

3.2.5锰过氧化物酶活性分析及其与纤维板强度的关联度比较 20

3.2.6总结 21

3.3木质纤维素组分的含量变化及其与纤维板强度的关联度比较 21

3.3.1木质素含量变化及其与纤维板强度的关联度比较 21

3.3.2纤维素含量变化及其与纤维板强度的关联度比较 22

3.3.3半纤维素含量变化及其与纤维板强度的关联度比较 23

3.3.4总结 24

4. 结论与展望 25

4.1结论 25

4.2展望 25

5 致谢 26

参考文献 27

附:本科期间取得的与毕业论文相关的成果 30

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